Education and training:
Dr. Ravi Gandham completed B.V.Sc. & A.H. at College of Veterinary Science, Tirupati, Andhra Pradesh Agricultural University; M.Sc. (Dairying) in Animal Genetics and Breeding from National Dairy Research Institute, Karnal; and PhD in Animal Genetics from Indian Veterinary Research Institute, Izatnagar, Bareilly. He underwent training at School of Informatics, Indiana University Purdue University Indianapolis, USA in the field of Computational Biology and Genomics. He joined NIAB on 24 May 2018.
Research experience and interests:
Dr. Gandham is experienced in the field of Animal Genomics and Computational Biology. He is a combination of being a computational and experimental biologist. He has handled several externally funded projects. His research interests at NIAB are host-pathogen interaction, Livestock Genomics and Functional studies. He works on host pathogen interaction studies to delineate molecular pathogenesis using high throughput genomics and proteomics approaches.The signature molecules identified in these studies will be confirmed through CRISPR/Cas9 knockout studies. He also works on livestock genomics. Currently, he’s is involved in the development of SNP chip for indigenous breeds of cattle and in the assembly of Indigenous cattle genome.
Selected awards, honours and fellowships:
1. ICAR Hari Om Ashram Trust Team National Award – 2017
2. ICAR – IVRI Best Teacher award for the year – 2015-16
3. FM Burnett Team Award -Indian Society of Veterinary Immunology & Biotechnology (ISVIB) – 2011
4. Asian Scientist Bursary award and DBT Financial Assistance for presenting a research paper at Tokyo – 2004
5. Junior and Senior Research fellowship of Indian Council of Agricultural Research for the Ph.D Programme – 1995 & 1997
India has a wide variety of indigenous cattle breeds distributed throughout its agro climatic zones. These are known for their natural tolerance to tropical heat and disease resistance with sustainable milk production. To meet the growing demand of milk in India several crossbreeding programmes were taken up. Every state has its own crossbreeding policy which is agro climatic and breed specific. This resulted in breed dilution of valuable indigenous cattle in their native breeding tract leaving behind very few purebreds in comparison to graded cattle. Also, our indigenous breeds have not been exploited to their potential because of slow genetic gain through generations using conventional breeding policies. With the crossbreds not withstanding harsh climate, being susceptible to tropical diseases and requiring constant input of good managemental conditions, conserving our indigenous cattle genetic resources which are far superior in these aspects is the need of the hour. To strike a balance between the increasing demand for milk and change in environment due to global warming conserving our indigenous cattle breeds becomes all the more important. For conserving the breeds, identification of purebreds is a major limitation. The admixture analysis to identify the purebreds is normally done using SNPchip(s). Further, though there are several Bovine SNP Chips available in the market, literature shows that these chips would partially cover the Bos indicus genome and that the available SNP chips truly do not represent our indigenous breeds. The availability of high-density chip with improved mapping resolution across indigenous breeds would further result in better imputation.
JE – Human vs Pig
Viremia is caused by the replication of viruses which results in viruses being introduced into the bloodstream. Domestic pigs are the major amplifying host for Japanese encephalitis (JE) virus transmission to humans, because they develop high titers and long-lasting viremia after natural infection. Humans, horses, and other non-avian vertebrates are considered dead-end hosts because they do not produce a level of viremia sufficient to infect new mosquitoes. With high titers of virus in the blood, pigs rarely show symptoms and humans with low levels of the virus in blood are affected. This differential host response will be explored by transcriptome (mRNA, miRNA, lncRNA, epitrancriptome and Circular RNA) and proteome analysis in the project that is taken up at our Institute.
Heat stress – Tharparkar vs Vrindavani (Crossbred)
In India, Tharparkar is one among the best dairy breeds. It is adapted to the Indian states of Punjab and Haryana. It is considered to be the hardiest, disease resistant, heat tolerant and tick resistant indigenous cattle breed of the country. This breed has also been used in several crossbreeding programs. Currently, percentage of purebreds is exceptionally low in India (Department of Animal Husbandry & Dairying, Govt. of India). Most of the farmer’s in India have Crossbreds and the percentage of exotic inheritance in these Crossbreds is unknown. Vrindavani, a Crossbred (synthetic), with 27% of Indigenous blood and 73% of exotic inheritance is a representation of the kind of admixture that prevails in Indian cattle. Therefore, comparing Tharparkar with Vrindavani may establish the lost importance of Indigenous cattle and would further emphasize the need of conserving our indigenous purebreds because of advantageous traits like Heat tolerance. Studies explaining the difference between the genetic groups (Crossbreds and Indigenous cattle) have been done mainly to address the physiological responses vis – a – vis heat stress and very few studies at the genomic level have been taken up. In this study, Tharparkar and Crossbred – Vrindavani cattle were subjected to heat stress and blood samples were collected on 0th day and 7th day, as it is known that short term acclimation occurs around 5 – 6 days. The transcriptome of 7th day was compared and with 0th day in both the genetic groups to understand their differential response to heat stress.
1. Rao, P.L., Gandham, R.K. & Subbiah, M. Molecular evolution and genetic variations of V and W proteins derived by RNA editing in Avian Paramyxoviruses. Sci Rep 10, 9532 (2020). https://doi.org/10.1038/s41598-020-66252-x
1. Wani S A, Sahu A R, Khan R I N, Pandey A, Saxena S, Hosamani N, Malla W A, Chaudhary D, Kanchan S, Sah V, Rajak K K, Muthuchelvan D, Mishra B, Tiwari A K, Sahoo A P, Sajjanar B, Singh Y P, Gandham R K, Mishra B P, Singh R K. Contrasting Gene Expression Profiles of Monocytes and Lymphocytes From Peste-Des-Petits-Ruminants Virus Infected Goats. Front Immunol 2019; 10:1463.
2. Pesingi P V, Singh B R, Pesingi P K, Bhardwaj M, Singh S V, Kumawat M, Sinha D K, Gandham R K. 2019. MexAB-OprM Efflux Pump of Pseudomonas aeruginosa Offers Resistance to Carvacrol: A Herbal Antimicrobial Agent. Front Microbiol. 2019 Nov 19;10:2664
3. Kundave V R, Ram H, Shahzad, M, Garg R, Banerjee P S, Nehra A K, Rafiqi S I, Ravikumar G, Tiwari A K. Genetic characterization of Theileria species infecting bovines in India. Infect Genet Evol 2019; 75:103962.
4. Manjunath S, Saxena S, Mishra B, Santra L, Sahu A R, Wani S A, Tiwari A K, Mishra B.P, Singh R K, Janga SC, Kumar G R. Early transcriptome profile of goat peripheral blood mononuclear cells (PBMCs) infected with peste des petits ruminant’s vaccine virus (Sungri/96) revealed induction of antiviral response in an interferon independent manner. Res Vet Sci 2019; 124:166-77.
5. Sinha R, Sahoo N R, Shrivastava K, Kumar P, Qureshi S, De UK, Kumar A, Kumar G., Bhushan, B. Resistance to ETEC F4/F18-mediated piglet diarrhoea: opening the gene black box. Trop Anim Health Prod 2019; 51:1307-20.
6. Bhatt, M., K. K. Rajak, S. Chakravarti, A. K. Yadav, A. Kumar, V. Gupta, V. Chander, K. Mathesh, S. Chandramohan, A. K. Sharma, K. Mahendran, M. Sankar, D. Muthuchelvan, R. K. Gandham, M. Baig, R. P. Singh and R. K. Singh (2019). “Phylogenetic analysis of haemagglutinin gene deciphering a new genetically distinct lineage of canine distemper virus circulating among domestic dogs in India.” Transbound Emerg Dis 66(3):1252
7. Pandit, A. A., R. K. Gandham, C. S. Mukhopadhyay, R. Verma and R. S. Sethi (2019). “Transcriptome analysis reveals the role of the PCP pathway in fipronil and endotoxin-induced lung damage.” Respir Res 20(1): 24.
1. Pantawane, P. B., H. Dhanze, G. Ravi Kumar, R. G. M, N. C. Dudhe and K. N. Bhilegaonkar (2018). “TaqMan real-time RT-PCR assay for detecting Japanese encephalitis virus in swine blood samples and mosquitoes.” Anim Biotechnol: 1
2. Wani, S. A., A. R. Sahu, S. Saxena, K. K. Rajak, M. Saminathan, A. P. Sahoo, S. Kanchan, A. Pandey, B. Mishra, D. Muthuchelvan, A. K. Tiwari, B. P. Mishra, R. K. Singh and R. K. Gandham (2018). “Expression kinetics of ISG15, IRF3, IFNgamma, IL10, IL2 and IL4 genes vis-a-vis virus shedding, tissue tropism and antibody dynamics in PPRV vaccinated, challenged, infected sheep and goats.” Microb Pathog 117: 206
3. Khanduri, A., A. R. Sahu, S. A. Wani, R. I. N. Khan, A. Pandey, S. Saxena, W. A. Malla, P. Mondal, K. K. Rajak, D. Muthuchelvan, B. Mishra, A. P. Sahoo, Y. P. Singh, R. K. Singh, R. K. Gandham and B. P. Mishra (2018). “Dysregulated miRNAome and Proteome of PPRV Infected Goat PBMCs Reveal a Coordinated Immune Response.” Front Immunol 9: 2631
4. Sahu, A. R., S. A. Wani, S. Saxena, K. K. Rajak, D. Chaudhary, A. P. Sahoo, A. Khanduri, A. Pandey, P. Mondal, W. A. Malla, R. I. N. Khan, A. K. Tiwari, B. Mishra, D. Muthuchelvan, B. P. Mishra, R. K. Singh and R. K. Gandham (2018). “Selection and validation of suitable reference genes for qPCR gene expression analysis in goats and sheep under Peste des petits ruminants virus (PPRV), lineage IV infection.” Sci Rep 8(1): 15969
5. Hussain, S., S. Saxena, S. Shrivastava, A. K. Mohanty, S. Kumar, R. J. Singh, A. Kumar, S. A. Wani, R. K. Gandham, N. Kumar, A. K. Sharma, A. K. Tiwari and R. K. Singh (2018). “Gene expression profiling of spontaneously occurring canine mammary tumours: Insight into gene networks and pathways linked to cancer pathogenesis.” PLoS One 13(12): e0208656.
1. Pandey, A., Sahu, A. R., Wani, S. A., Saxena, S., Kanchan, S., Sah, V., Rajak, K. K., Khanduri, A., Sahoo, A. P., Tiwari, A. K., Mishra, B., Muthuchelvan, D., Mishra, B. P., Singh, R. K. and Gandham, R. K. (2017) Modulation of Host miRNAs Transcriptome in Lung and Spleen of Peste des Petits Ruminants Virus Infected Sheep and Goats. Front Microbiol, 8, 1146.
2. Manimegalai J, Minakshi Prasad, Basanti Brar, Ikbal, Ravi Kumar Gandham, Upendra P Lambe, Koushlesh Ranjan and Jyoti Misri (2017) Validation of Interferon Induced IFIT Genes in Host Antiviral Defence Against BTV16 Infection : An Initial Report. The Indian Journal of Veterinary Sciences & Biotechnology (2017) Volume 13, Issue 1, 70-76.
3. Priya, G. B., Nagaleekar, V. K., Milton, A. A. P., Saminathan, M., Kumar, A., Sahoo, A. R., Wani, S. A., Gupta, S. K., Sahoo, A. P., Tiwari, A. K., Agarwal, R. K. and Gandham, R. K. (2017) Genome wide host gene expression analysis in mice experimentally infected with Pasteurella multocida. PLoS One, 12, e0179420.
4. R.S. Rajmani, Lakshya Veer Singh, Shishir Kumar Gupta, Prafull Kumar Singh, Shikha Saxena, G. Ravi Kumar, Rajiv Kumar, Aditya P. Sahoo, Ashok K. Tiwari (2017) Molecular cloning, expression and characterization of rat tumor necrosis factor-α as potent anti-tumor candidate. Gene Reports, 7, 113-118.
5. Sahu, A. R., Wani, S. A., Saminathan, M., Rajak, K. K., Sahoo, A. P., Pandey, A., Saxena, S., Kanchan, S., Tiwari, A. K., Mishra, B., Muthuchelvan, D., Singh, R. P., Singh, Y., Baig, M., Mishra, B. P., Singh, R. K. and Gandham, R. K. (2017) Genome sequencing of an Indian peste des petits ruminants virus isolate, Izatnagar/94, and its implications for virus diversity, divergence and phylogeography. Arch Virol, 162, 1677-1693.
6. Manjunath, S., Mishra, B. P., Mishra, B., Sahoo, A. P., Tiwari, A. K., Rajak, K. K., Muthuchelvan, D., Saxena, S., Santra, L., Sahu, A. R., Wani, S. A., Singh, R. P., Singh, Y. P., Pandey, A., Kanchan, S., Singh, R. K., Kumar, G. R. and Janga, S. C. (2017) Comparative and temporal transcriptome analysis of peste des petits ruminants virus infected goat peripheral blood mononuclear cells. Virus Res, 229, 28-40.
7. Singh, A., Prasad, M., Mishra, B., Manjunath, S., Sahu, A. R., Bhuvana Priya, G., Wani, S. A., Sahoo, A. P., Kumar, A., Balodi, S., Deora, A., Saxena, S. and Gandham, R. K. (2017) Transcriptome analysis reveals common differential and global gene expression profiles in bluetongue virus serotype 16 (BTV-16) infected peripheral blood mononuclear cells (PBMCs) in sheep and goats. Genom Data, 11, 62-72.
8. Kumar, A., Gaur, G. K., Gandham, R. K., Panigrahi, M., Ghosh, S., Saravanan, B. C., Bhushan, B., Tiwari, A. K., Sulabh, S., Priya, B., V, N. M., Gupta, J. P., Wani, S. A., Sahu, A. R. and Sahoo, A. P. (2017) Global gene expression profile of peripheral blood mononuclear cells challenged with Theileria annulata in crossbred and indigenous cattle. Infect Genet Evol, 47, 9-18.
1. Sajad Ahmad Wani, Amit Ranjan Sahu, Shikha Saxena, Shahid Hussain, Aruna Pandey,Sonam Kanchan, Aditya Prasad Sahoo, Bina Mishra, Ashok Kumar Tiwari, Bishnu Prasad Mishra, Ravi Kumar Gandham, Raj Kumar Singh (2016). Systems Biology approach: Panacea for unravelling host-virus interactions and dynamics of vaccine induced immune response. Gene Reports DOI : 10.1016/j.genrep.2016.08.002
2. Rajmani RS, Gupta SK, Singh PK, Gandham ravi Kumar, Sahoo AP, Chaturvedi U, & Tiwari Ashok Kumar (2016). HN protein of Newcastle disease virus senitizes HeLa cells to TNF-α-induced apoptosis by down regulating NF-κβexpression. Archives of Virology, 161 (9): 2395-2405
3. Shishir Kumar Gupta, Pavan Kumar Yadav, A.K. Tiwari, Ravi Kumar Gandham and AP Sahoo (2016). Poly (I:C) enhances the anti-tumour activity of canine parvovirus NS1 protein by inducing a potent anti-tumour immunes response. Tumour Biology, DOI10.1007/s13277-016-5093-z.
4. Singh M.N., Raina O.K., Sankar M., Rialch A., Tigga M.N., Kumar G.R. & Banerjee P.S. (2016) Molecular detection and genetic diversity of Babesia gibsoni in dogs in India. Infect Genet Evol 41, 100-6.
5. Shishir Kumar Gupta, Ashok K. Tiwari, Ravi Kumar Gandham and AP Sahoo (2016). Combined administration of apoptin gene and poly (I:C) induces potent anti-tumour immune response and inhibits growth of mouse mammary tumours. International Immunopharmacology, 35: 163-173
6. Ravi Kumar G, Shikha Saxena, Sahoo AP, Chaturvedi U, Satish Kumar, Lakshman Santra, Laksyaveer Singh and Ashok K Tiwari (2016). Production and characterization of monoclonal antibodies to Newcastle disease virus. Indian journal of Experimental Biology 54: 180.
7. Shishir Kumar Gupta, Pavan Kumar Yadav, Ravi Kumar Gandham, A.P. Sahoo, D. R. Harish, Arvind Kumar Singh, A.K. Tiwari (2016). Canine parvovirus NS1 protein exhibits anti-tumor activity in a mousemammary tumor model. Virus Research 213:289–298.
8. Shishir Kumar Gupta, Aditya Prasad Sahoo, Nighil Rosh, Ravi Kumar Gandham, Lovleen Saxena, Arvind Kumar Singh, Harish DR, Ashok Kumar Tiwari (2016). Canine parvovirus NS1 induced apoptosis involves mitochondria, accumulation of reactive oxygen species and activation of caspases. Virus Research 213:46-61
1. Amit Ranjan Sahu, Sajad Ahmad Wani, Arpita Padhy, Amod Kumar, Govindara jan, Bhuvana Priya, Aditya Prasad Sahoo, Ashok Kumar Tiwari, Ravi Kumar Gandham (2015) Host-Virus Interaction: Role of miRNA and Bioinformatics Tools for miRNA Target Prediction. Advances in Animal and Veterinary Sciences 3(4) :30-36.
2. Sajad Ahmad Wani, Amit Ranjan Sahu, Bishnu Prasad Mishra, Amod Kumar, Govindarajan Bhuvana Priya, Arp ita Padhy, Aditya Prasad Sahoo, Ashok Kumar Tiwari, Ravi Kumar Gandham, Raj Kumar Singh (2015) Whole Genome Sequence Analysis of Viruses; Moving Beyond Single/Partial Gene Based Phylogenies in Context of Epidemiology and Genetic Evolution. Advances in Animal and Veterinary sciences. 3(8) :435-443.
3. R. S. Rajmani, Ravi Kumar Gandham, Shishir Kumar Gupta, Aditya P. Sahoo, Prafull Kumar Singh, Shikha Saxena, Rajiv Kumar, Uttara Chaturvedi and AshokK(2015).Tiwari.Administration of IkB-kinase inhibitor PS1145 enhance apoptosis in DMBA-induced tumor in male Wistar rats.Cell Biol Int.9999:1-12.
4. R. S. Rajmani, Ravi Kumar Gandham, Shishir Kumar Gupta, Aditya Prasad Sahoo, Prafull Kumar Singh, Rajiv Kumar, Shikha Saxena, Uttara Chaturvedi & Ashok K. Tiwari (2015). HN Protein of Newcastle Disease Virus induces apoptosis through SAPK/JNK pathway. Appl Biochem Biotechnol. DOI 10.1007/s12010-015 -1788-7.
5. Manjunath S., Kumar G., Mishra B., Mishra B., Sahoo A., Joshi C.G., Tiwari A.K., Rajak K. & Janga S. (2015). Genomic analysis of host – Peste des petits ruminants vaccine viral transcriptome uncovers transcription factors modulating immune regulatory pathways. Vet Res 46, 15.
6. Siddappa Manjunath BM, Bishnu Prasad Mishra, Shikha Saxena, Piyali Mondal, Amit Ranjan Sahu, Aditya Prasad Sahoo, Ashok K. Tiwari, Ravi Kumar Gandham (2015). Identification of suitable reference gene in goat peripheral blood mononuclear cells (PBMCs) infected with peste des petits ruminants virus (PPRV). Livestock Science 181:15-155. DOI 10.1016/j.livsci.2015.09.010.
7. Garg, R., P. K. Patil, S. V. Singh, S. Sharma, R. K. Gandham, A. V. Singh, G. Filia, P. K. Singh, S. Jayaraman, S. Gupta, K. K. Chaubey, R. Tiwari, M. Saminathan, K. Dhama and J. S. Sohal (2015).Comparative Evaluation of Different Test Combinations for Diagnosis of Mycobacterium avium Subspecies paratuberculosis Infecting Dairy Herds in India. Biomed Res Int 2015: 983978.
8. Singh, P. K., A. K. Tiwari, R. S. Rajmani, G. R. Kumar, U. Chaturvedi, L. Saxena, S. Saxena, J. Doley, A. P. Sahoo, L. Santra, M. Saxena, S. Kumar and B. Sharma (2015). Apoptin as a potential viral gene oncotherapeutic agent. Appl Biochem Biotechnol 176(1): 196-212.
9. Dhanze H., Bhilegaonkar K.N., Ravi Kumar G.V., Thomas P., Chethan Kumar H.B., Suman Kumar M., Rawat S., Kerketta P., Rawool D.B. & Kumar A. (2015) Comparative evaluation of nucleic acid-based assays for detection of Japanese encephalitis virus in swine blood samples. Arch Virol 160, 1259-66.
10. Harish D. Ramachandra, Arvind K. Singh, Shishir K. Gupta, Madhu C. Lingaraju, Goravanahalli M. Vidyalakshmi, Aditya P. Sahoo, G. Ravi Kumar and Ashok K. Tiwari. (2015). Matrix Metallo Proteinases Activities in N-methyl-N-nitrosourea Induced Mammary Tumour in Wistar Rats. J Pure Applied Microbiology, 3
11. Harish D. Ramachandra, Arvind K. Singh, Shishir K. Gupta, Madhu C. Lingaraju, Goravanahalli M. Vidyalakshmi, Aditya P. Sahoo, G. Ravi kumar and Ashok K. Tiwari. (2015). Identification of Mammary Tumour Homing Peptides by In vivo Biopanning using Phage Display Library. J Pure Applied Microbiology, 3.
12. R. S. Rajmani, Ravi Kumar, Shishir Kumar Gupta, Aditya P. Sahoo, Prafull Kumar Singh, Shikha Saxena, Rajiv Kumar, Uttara Chaturvedi and Ashok K. Tiwari (2015). Administration of IkB-kinase inhibitor PS1145 enhances apoptosis in DMBA-induced tumor in male Wistar rats. Cell Biology International, 9999 (2015) 1–12.
13. Shikha Saxena, GS Desai, G Ravi Kumar, AP Sahoo, Lakshman Santra, Lakshya Veer Singh, Sudesh Kumar & AK Tiwari (2015). Characterization and evaluation of apoptotic potential of double gene construct pVIVO.VP3.NS1. Indian Journal of Experimental Biology, 53: 249-255.
14. Gupta S.K., Gandham R.K., Sahoo A.P. & Tiwari A.K. (2015) Viral genes as oncolytic agents for cancer therapy. Cell Mol Life Sci 72, 1073-94.
15. Sahoo A.P., Tiwari A.K., Ravi Kumar G., Chaturvedi U., Singh L. V., Saxena S., Palia S.K., Jadon N.S., Singh R., Singh K.P., Brahmaprakash B.S., Maiti S.K. & Das A.K. (2015) Establishment and characterization of a bovine rectal myxoma cell line. Tissue & Cell 47, 49-54.
16. Rajmani R.S., Singh P.K., Ravi Kumar G., Saxena S., Singh L.V., Kumar R., Sahoo A.P., Gupta S.K., Chaturvedi U. & Tiwari A.K. (2015) In-vitro Characterization and Evaluation of Apoptotic Potential of Bicistronic Plasmid Encoding HN Gene of Newcastle Disease Virus and Human TNF-alpha. Anim Biotechnol 26, 112-9.
1. R S Rajmani, Prafull Kumar Singh, Sanjay Kumar, G Ravi Kumar, Aditya P Sahoo, Lakshman Santra, Shikha Saxena, Lakshya Veer Singh, Uttara Chaturvedi, Lovleen Saxena, G S Desai, Shishir Kumar Gupta, Amit Kumar, N S Jadon & Ashok K Tiwari (2014). Development of dog mammary tumor xenograft in immunosuppressed Swiss albino mice. Indian J. Exp. Biology, 52: 935-42. NAAS=6.84
2. Mandal M, Banerjee PS, Garg R, Ram H, Kundu K, Kumar S, Kumar GV. 2014. Genetic characterization and phylogenetic relationships based on 18S rRNA and ITS1 region of small form of canine Babesia spp. from India. Infect Genet Evol 27: 325-331.
3. Thadiyam Puram Ramees, Ramswaroop Singh Rathore, Prashanth Suresh Bagalkot, G.V.P.P.S. Ravi Kumar, Hosakote Venkatappa ,Mohan1, R. Anoopraj, Ashok Kumar and Kuldeep Dhama (2014) Real-Time PCR Detection of Arcobacter butzleri and Arcobacter cryaerophilus in Chicken Meat Samples. Journal of pure and applied microbiology, 8(4), p. 3165-3169.
4. Hitesh N Pawar, G Ravi Kumar, Raman Narang and Ravi Kant Agrawal 2014. Heat and cold stress enhances the expression of heat shock protein 70, heat shock transcription factor 1 and cytokines (IL-12, TNF- and GMCSF) in buffaloes. Int.J. Curr. Microbiol.App. Sci 3: 307-317.
5. Santra L, Rajmani RS, Kumar GV, Saxena S, Dhara SK, Kumar A, Sahoo AP, Singh LV, Desai GS, Chaturvedi U et al. 2014. Non-Structural protein 1 (NS1) gene of Canine Parvovirus-2 regresses chemically induced skin tumors in Wistar rats. Res Vet Sci 97: 292-296.
6. Lakshman Santra, Rajmani RS, G.V.P.P.S. Ravi kumar, Sujoy Kumar Dhara, Shikha Saxena, A P Sahoo, G S Desai, Lakshya Veer Singh, Uttara Chaturvedi, Sudesh Kumar and Ashok K Tiwari. . 2014. In vitro cloning of Canine Parvo virus NS1 gene and reporter gene GFP in eukaryotic expression vector pVIVO-mcs and characterization of double gene construct in mammalian cells. Indian Journal of Biotechnology 13: 41-46.
7. Manjunath Siddappa, Ravi Kumar G, Vishal Sarsani, Bishnu Prasad Mishra, Bina Mishra, C. G. Joshi, A. P. Sahoo, A. K. Tiwari, Sarath Chandra Janga. 2014. Whole-Genome Sequence of Sungri/96 Vaccine Strain of Peste des Petits Ruminants Virus. Genome Announc 2.
8. Lakshya Veer Singh, Shikha Saxena, Smita Gupta, Shishir Kumar Gupta, G. Ravi Kumar, G. S. Desai, Aditya Prasad Sahoo, Harish DR and A. K.Tiwari. 2014. Evaluation and comparison of the constitutive expression levels of Toll-like receptors 2, 3 and 7 in the peripheral blood mononuclear cells of Tharparkar and Crossbred cattle from different regions of India. Veterinary World 7:209-212.
9. Doley J, Singh LV, Kumar GR, Sahoo AP, Saxena L, Chaturvedi U, Saxena S, Kumar R, Singh PK, Rajmani RS et al. 2014. Canine parvovirus type 2a (CPV-2a)-induced apoptosis in MDCK involves both extrinsic and intrinsic pathways. Appl Biochem Biotechnol 172: 497-508.
10. Das DP, Malik SV, Rawool DB, Das S, Shoukat S, Gandham RK, Saxena S, Singh R, Doijad SP, Barbuddhe SB. 2014. Isolation of Coxiella burnetii from bovines with history of reproductive disorders in India and phylogenetic inference based on the partial sequencing of IS1111 element. Infect Genet Evol 22: 67-71.
1. Saxena L., Kumar G.R., Saxena S., Chaturvedi U., Sahoo A.P., Singh L.V., Santra L., Palia S.K., Desai G.S. & Tiwari A.K. (2013) Apoptosis induced by NS1 gene of Canine Parvovirus-2 is caspase dependent and p53 independent. Virus Res 173, 426-30.
2. Swati, GVPPS Ravi Kumar, Gurvinder Singh Brah, Dipak Deka, R. K. Agrawal, Meera D Ansal and C S Mukhopadhyay (2013). Differential expression profiling of toll like receptors 3, 4 and 9 genes in major tissues of Indian major carp Catla catla. 83 (9): 983–986, Indian Journal of Animal Sciences.
3. Uttara Chaturvedi, G. Ravi Kumar, G. Desai, Sudesh kumar, Shahina Kalim, Aditya P Sahoo, B.B. Dash, Sangeeta Tiwari, Barkha Ratta, Lakshya Veer Singh and Ashok. K. Tiwari (2013), Phylogenetic and pathogenic analysis of Indian isolates of Newcastle Disease Virus. Indian Journal of Biotechnology 12:425-428.
4. Hitesh N. Pawar, G Ravi Kumar and Raman Narang. 2013. Effect of Heat Stress on Milk Production and Composition in Murrah Buffaloes Journal of Buffalo Science 2: 98-102.
5. Uttara Chaturvedi, Shahina Kalim, L. V. Singh, A. P. Sahoo, S. K. Palia, Rajiv Kumar, G. Ravi Kumar, Sangeeta Tiwari, Ashok K. Tiwari 2013. Cloning, expression and phylogenetic analysis of IFN-𝛄 gene in chicken. Indian J of animal Science 83 (4): 398-401.
6. Kumar R, Tiwari AK, Chaturvedi U, Kumar GR, Sahoo AP, Kumar S, Tiwari S. 2013. Cloning and expression analysis of multiple proteins encoding P gene of Newcastle disease virus. Indian J Exp Biol 51: 116-123.
1. Kumar R, Tiwari AK, Chaturvedi U, Kumar GR, Sahoo AP, Rajmani RS, Saxena L, Saxena S, Tiwari S, Kumar S. 2012. Velogenic newcastle disease virus as an oncolytic virotherapeutics: in vitro characterization. Appl Biochem Biotechnol 167: 2005-2022.
2. Singh P.K, Doley J, Kumar G.R., Sahoo A.P., Tiwari A.K. (2012). Oncolytic Viruses and their specific targeting to tumour cells. Indian Journal of Medical Research,136 : 571-584.
3. Saxena S, Kumar GR, Singh P, Chaturvedi U, Saxena L, Kumar R, Sahoo AP, Doley J, Rajmani, Kumar A et al. 2012. Prokaryotic expression of chicken infectious anemia apoptin protein and characterization of its polyclonal antibodies. Indian J Exp Biol 50: 325-331.
4. Shah SM, Ravi Kumar GV, Brah GS, Santra L, Pawar H. 2012. Differential Expression of Th1- and Th2- Type Cytokines in Peripheral Blood Mononuclear Cells of Murrah Buffalo (Bubalus Bubalis) on TLR2 Induction by B. Subtilis Peptidoglycan. Asian-Australas J Anim Sci 25: 1021-1028.
5. Kumar G.R., Saxena S., Saxena L., Chaturvedi U., Santra L., Kumar R., Sahoo A.P., Rajmani, Kumar A., Desai G.S., Kumar S. & Tiwari A.K. (2012) In vitro expression studies of non structural 1 protein of Canine Parvo virus 2 by polyclonal antiserum raised against CPV2-NS1 protein expressed in Escherichia coli as an antigen. Indian J Exp Biol 50, 618-24.
6. G.V.P.P.S Ravi Kumar, V. Vohra, Aditya.P.Sahoo, Lakshman Santra and Shikha Saxena (2012) Whole genome selection: An overview Journal of Animal Research 2(1):1-8.
1. Srinivasa Rao T., J.P.S. Gill, G.V.P.P.S. Ravi Kumar, Sandeep Ghatak, K.N. Bhilegaonkar Mudasir Ali Rather and P.K. Patil (2011). Prevalence and Putative Virulence Genes of Shiga Toxin-Producing Escherichia Coli Isolated from Water, Fish and Human Diarrhoeic Samples in Punjab, India. International Journal of Applied Biotechnology and Biochemistry. 1: 59-70
2. Rajmani R.S., Doley J., Singh P.K., Kumar Ravi, Barathidasan R., Kumar Pawan, Verma P.C. and Tiwari A.K. 2011. Induction of skin tumour using DMBA in wistar Rat and histopathological evaluation. Indian J. Vet. Pathol. 35(2) : 217-220.
3. Rajmani R.S., Doley J., Singh P.K., Kumar Ravi, Barathidasan R., Kumar Pawan, Verma P.C. and Tiwari A.K. 2011. Induction of mammary gland tumour in rats using N-methyl-N-nitroso urea and their histopathology. Indian J. Vet. Pathol. 35(2) : 142-146, 2011.
4. Saxena L, Chaturvedi U, Saxena S, Kumar GR, Sahoo AP, Kumar S, Doley J, Rajmani RS, Singh PK, Kumar R et al. 2011. Characterization and in vitro expression of non-structural 1 protein of canine parvovirus (CPV-2) in mammalian cell line. Indian J Exp Biol 49: 654-659.
1. GVPPS. Ravi Kumar, H.N.Pawar, G.S.Brah, Syed M.Shah, M.L.Chaudhary and C.S. Mukhopadhyay (2010) Expression pattern of TLR-7 in chicken tissues by semi-quantitative PCR using experion automated capillary electrophoresis system. Indian Journal of Poultry Science. 45(3): 345-347
1. A.Chattopadhyay, G.Ravi Kumar, M.L.Chaudhary and G.S. Brah (2009).Genetic Diversity analysis in chickens using Microsatellite Markers. Indian Journal of Poultry Science. 44(2):163-166
2. Satyendra Pal singh, G.S. Brah, M.L. Chaudhary and G.V.P.P.S. Ravi Kumar.(2009).Evaluation and Comparison of Immune Response to Sheep Red Blood Cells and PHA-P Inoculation in pure and cross bred Chickens. Indian Journal of Poultry Science. 44(1):21-24
3. Satyendra Pal Singh, G.V.P.P.S. Ravi Kumar and G.S. Brah. (2009). Genetic diversity analysis in egg type chickens using microsatellite Markers. Indian Journal of Animal Science. 79(5):519-520. Impact Factor – 0.16
4. Mavi,P.S.,Bagha,C.S.,Ravi Kumar, G.V.P.P.S. Uppal,S.K. and Sidhu,S.S.(2009). Efficacy of an antistress herbal preparation on milk production in FMD vaccinated dairy cows and buffaloes. Indian Veterinary Journal, 86(4):395-396
5. Das, K.S., Das, N. and Ravi Kumar, G.V.P.P.S. (2009). Effect of extra concentrate feeding on reproduction in dairy cows. Indian Veterinary Journal, 86(11):1197-1198
6. G.V.P.P.S.Ravi Kumar, G.S.brah, M.L.Chaudhary, H.N.Pawar and Pranav Mathur(2009). Cloning, Sequencing and Characterization of chicken growth hormone receptor gene. Indian Journal of Poultry Science. 44(1):15-20
1. Ravi Kumar, G.V.P.P.S., Amrita.C., Brah. G.S. and Chaudhary M.L. (2008).PCR-RFLP and nucleotide sequencing of neuropeptide Y (NPY) gene in egg type chickens. Indian Journal of Poultry Science. 43(3):263-266
2. Tanman kaur., Ravi Kumar, G.V.P.P.S., Bajwa I.S. and Trehan.P.K. (2008). PCR-RFLP of Growth hormone gene in meat-type chickens. Indian Journal of Poultry Science, 43(2):129-133
3. Ravi Kumar, G.V.P.P.S., Sharma, A., Suryanarayana,V.V.S. and Ravi Kumar, P. (2008). Sequence characterization and genetic variation of Bos indicus Glucose-6-phosphate-dehydrogenase gene. DNA sequence, 19(1):37- 43.
4. Satyendra Pal Singh., Brah, G.S., Amrita, C. and Ravi kumar, G.V.P.P.S. (2008). PCR-RFLP of chicken growth hormone gene in egg-type chickens. Indian Journal of Poultry Science,43(1):1-4
1. Samita Saini., Chaudhary, M.L., Brah, G.S. and Ravi Kumar, G.V.P.P.S. (2007). Polymorphism analysis in egg type chicken using Microsatellite Markers. Indian Journal of Poultry Science, 42(1):27-30
2. Simarjeet Kaur., Parmar, O S. and Ravi Kumar, G.V.P.P.S. (2007). RAPD Assay to evaluate the genetic diversity of Holstein-Friesian crossbred cattle produced using sires obtained from different countries. Indian journal of Dairy Science,60(4): 268-273
3. Ravikumar, G., Satyendra, P.S., Amrita, C., and Kaur, T.(2007). Functional characterization of catalytic site of Glucose-6-phosphate dehydrogenase gene in Bos indicus. Online Journal of Veterinary Research,11(1): 20-29
4. Das, K.S., Das, N. and Ravi Kumar, G.V.P.P.S. (2007). Effect of Steaming up on subsequent production in dairy cows. Indian Journal of Animal Science,77(7):583-585. Impact Factor – 0.16
5. Srikala, R., Ravikumar, G., Satyendra, P.S., Amrita, C. and Rajni, G.(2006).Variability in the intron-2 of leptin gene in Reverine buffaloes. Online Journal of Veterinary Research,10(2): 146-155
1. Kumar,R., Kaur, R., Kaur,S., Sethi, P. and Srikala, R. (2006). Sequence characterization and phylogenetic analysis of Glucose-6-phosphate-dehydrogenase gene in Bubalis bubalus. Online Journal of Veterinary Research, 10(2): 116-120.
2. Rajvinder Kaur., Mehra, M.L.and Ravi Kumar, G.V.P.P.S. (2006). Random polymorphic DNA markers to Assess inbreeding in buffaloes. Journal of Research (PAU),43(2):237-240
3. Singh,S.T., Parmar, O.S., Ravi Kumar, G.V.P.P.S., Uppal, S.K. and Sharma.S. (2006). Mortality pattern in relation to age, Season, birth, weight, sex and diseases in crossbred cattle. Veterinary Practitioner,7(2):117-119
1. Parmar.,O.S and G.V.P.P.S.Ravi Kumar.(2004).A new approach for conservation of elite germplasm of dairy animals in Punjab. Indian Journal of Animal breeding and Genetics,25(1),35-38
1. Ravi kumar, G.V.P.P.S., Sharma, A. and Narang, R. (2003). Biochemical studies of glucose-6-phosphate-dehydrogenase in Tharparkar cattle. SARAS journal of livestock and poultry production, 19(3-4):53-58. Impact Factor – 1.76
2. Ravi kumar, G.V.P.P.S. and Parmar,O.S. (2003).Serum protein profile studies in crossbred cattle. SARAS journal of livestock and poultry production,19 (3-4): 59-60
1. Parmar., O.S, Joshi., B.K. and Ravi kumar., G.V.P.P.S. (1999). Conservation and management of cattle genetic resources. Indian Journal of Animal breeding and Genetics,1(1),114-128
1. Ravi Kumar, G.V.P.P.S. and Ashwini Sharma (1998). Phenotypic alterations in goats fed subabul (Leucaena luecocephala). Indian Journal of Animal Science, 68(4):402- 404.Impact Factor – 0.16
1. Ashwini Sharma and Ravi Kumar, G.V.P.P.S. (1997). Genotoxic studies on subabul (Leucaena leucocephala). Subabul: A promising feed for goats. Livestock International, 1(7):6-10
2. Ravi Kumar, G.V.P.P.S. and Ashwini Sharma. (1997). Genotoxic effects of Subabool (Luecaena leucocephala) in goats. Indian Journal of Dairy Science, 50(50):376-383
Monographs and Technical Bulletins
1. Developed a monograph on “Phylogenetic analysis using MEGA 4.0, MEGA 5.05 and PHYLIP”
2. Developed a technical Bulletin – “Analysis of Real time PCR”
1. For “National workshop – cum – training programme on “Bioinformatics tools for genome analysis” held from March 14-16, 2011 at Bioinformatics centre, IVRI, 1-91
2. In silico approach for genome analysis. (2012). Eds, A.K.Tiwari, G Ravi Kumar, A P Sahoo and GS Desai, IVRI, Izatnagar, 1-156
3. Bioinformatics tools in Biotechnology (2012).Eds, A.K.Tiwari, G Ravi Kumar, A P Sahoo and GS Desai, IVRI, Izatnagar, 1-194
4. Computational Approaches in Biotechnology for Beginners (2014).Eds, GVPPS Ravi Kumar, AP Sahoo, GS Desai, Ravikant Agarwal, & A. K. Tiwari, IVRI, Izatnagar, 1-210
5. Basic Concepts in Bioinformatics for beginners (2015).Eds Ravi Kumar Gandham, Aditya P Sahoo, Ravi Kant Agarwal and A.K.Tiwari, IVRI, Izatnagar, 1-198
6. Analysis of high throughput Sequencing and Microarray data to unravel Host-Pathogen Interactions (2015). Eds. Ravi Kumar Gandham, Aditya P Sahoo and Amit Kumar, IVRI, Izatnagar, 1-194
7. Bioinformatics for Beginners (2017). Eds. Ravi Kumar Gandham, Aditya P Sahoo, Ravi Kant Agrawal and A.K.Tiwari. IVRI, Izatnagar, 1-180
8. Real Time PCR data analysis (2018). Eds. G Sai Kumar, Himani Dhanze,Deepak Kumar,Ravi Kant Agrawal,K.N.Viswas and Ravi Kumar Gandham IVRI, Izatnagar, 1 – 78
Dr. Neelima Hosamani
PhD in Biochemistry, Yogi Vemana University, Andhra Pradesh
Current research interest- Genome sequence and Genetic diversity analysis to know the transmission of sheep poxvirus and goat poxvirus
Manas Ranjan Praharaj
PhD Scholar (CSIR-JRF)
MVSc in Animal Biotechnology, Indian Veterinary Research Institute, Bareilly, Uttar Pradesh
Current research interest-Elucidating the mechanism of Japanese Encephalitis Virus (JEV) infection in pig:amplifier host and human:dead-end host through Omics: Transcriptomics, Proteomics and Epitranscriptomics
PhD Scholar (CSIR-JRF)
M.Sc in Biotechnology, GITAM University, Visakapatnam, Andhra Pradesh
Current research interest: Role of non-structural protein(s) and epigenetic regulation in Japanese Encephalitis Virus (JEV) infection
BSc in Microbiology, Zoology & Chemistry, Mahatma Gandhi University, Nalgonda, Telangana
1. Role of Investigator: PI
Title of the Project: Genomics for conservation of indigenous cattle breeds and for enhancing milk yield, Phase-I
Funding Agency: DBT
Period/Duration: 2018 – Ongoing
2. Role of Investigator: Co-PI
Title of the Project: Unique traits in indigenous livestock making them resilient to climate change and database development: impact of climate variability on major zoonotic stresses including surveillance (livestock disease resistance traits)
Funding Agency: ICAR
Period/Duration: 2014 – Ongoing
3. Role of Investigator: PI
Title of the Project: Genomics assisted pathobiology to identify novel targets for diagnosis and therapeutic intervention(s) of Japanese Encephalitis and Leptospirosis
Funding Agency: DBT
Period/Duration: 2019 – Ongoing
Externally funded projects (Completed):
1. Role of Investigator: Elucidating the gene network(s) and identification of novel gene(s) underlying host-pathogen interaction in PPRV infection
Title of the Project: PI
Funding Agency: DBT
Period/Duration: 2013 -2016
2. Role of Investigator: Co-PI
Title of the Project: Identification of viral gene and development of tumour targeted nano-delivery vehicle for cancer therapy in Bovines
Funding Agency: NAIP
Period/Duration: 2011 – 2014
3. Role of Investigator: PI
Title of the Project: Understanding the molecular events associated with economically important diseases of livestock through system biology approach
Funding Agency: ICAR – IASRI
Period/Duration: 2014 – 2018
Institute funded projects (Completed):
4. Role of Investigator: Co-PI
Title of the Project: Prediction of breed composition and genetic diversity in Vrindavani crossbred cattle
Funding Agency: IVRI
Period/Duration: 2016 – 2019
5. Role of Investigator: PI
Title of the Project: Development of monoclonal antibodies against Newcastle disease Virus
Funding Agency: IVRI
Period/Duration: 2010 – 2013
6. Role of Investigator: PI
Title of the Project: Studies on Growth hormone gene polymorphism in meat and egg type chicken
Funding Agency: PAU
Period/Duration: 2004 – 2006
7. Role of Investigator: PI
Title of the Project: Studies on Growth hormone receptor and NPY genes polymorphism in egg type chicken
Funding Agency: PAU
Period/Duration: 2006 – 2008
8. Role of Investigator: Co-PI
Title of the Project: Molecular and Cytogenetic Studies in farm animals (PV-3)
Funding Agency: PAU
Period/Duration: 2003 – 2005
9. Role of Investigator: Co-PI
Title of the Project: Molecular and Cytogenetic Studies on animals for faster genetic gains (NPV-61)
Funding Agency: PAU
Period/Duration: 2005 – 2007
National Institute of Animal Biotechnology
Survey No. 37, Opp. Journalist Colony
Extended Q City Road, Near Gowlidoddy
Telangana – 500032
1. Genome assembly of viruses – PPR, Equine Herpes Virus and Sheeppox; and Bacteria – Brucella melitensis and Mycobacterium bovis
2. Systems Biology behind heat tolerance in Tharparkar predicted
3. Contrasting gene expression profiles identified in monocytes and lymphocytes from goats infected with peste-des-petits-ruminants virus
4. Key differences in PRRV infected sheep and goat identified-