Dr. Paresh Sharma received his PhD (2010) from BITS Pilani, India. He did his Post-doctoral work in Malaria Biology at the NIAID, National Institute of Health (NIH), USA from 2010-2013. He joined the National Institute of Animal Biotechnology in 2013.
Dr. Paresh’s has extensive research experience in the field of Microbiology, Cell Biology, Immunology and Genomics. His research interest has been focused on understanding the molecular mechanisms behind major intracellular parasitic diseases for identifying new drug treatments, diagnostic tools, and vaccines. During his PhD and Postdoctoral work he has used genetic and genomic approaches to study the host parasite interaction and molecular mechanisms using the protozoan parasites Plasmodium falciparum, Leishmania donovani and Trypanosma brucei as a model.
Google Scholar Link:
https://scholar.google.co.in/citations?user=2tTBQ5MAAAAJ&hl=en
Figure1: Host signalling pathways involved during the apicomplexan parasite infection.
Host Parasite Interaction studies in Theileria-transformed Bovine Leukocytes having Cancer Hallmarks One of the economically important parasitic disease is Tropical Theileriosis, caused by the Apicomplexan parasite Theileria annulata. In India the economic loss due this disease is estimated at Rs. 8426.7 crores in 2017.The uniqueness of this apicomplexan lies in the fact that it transforms the host cells post infection and the hallmarks of transformations are similar to cancer. We are working to elucidate the fascinating biology of the parasite with a purpose to develop novel intervening strategy (Vaccine/Diagnostic Tool/Novel drug) using a holistic approach by combining data from genomics, epigenetics and proteomics. We have successfully characterized the parasite population structure of India by using microsatellite markers. We have developed RT-PCR based assay to quantify the host to parasite ratio, which will help in monitoring parasite load and treatment in animals. Presently our team is working towards identifying the host and parasite signalling pathways that plays a key role in disease pathogenesis and validating the same by using modern gene editing tool like CRISPR. We are also interested in screening and development of new anti-theilerial drugs.Figure2: Current research focus of our lab.
Antimicrobial Resistance Antimicrobial Resistance (AMR) is one of the major global problem associated with animal and human health. One health approach to solve the problem is required, therefore in our lab we are looking for new drug targets, repurposing of the available drugs and understanding the drug resistance mechanisms of the resistant pathogens. So far we’ve successfully repurposed the compounds like MMV656501, MMV687807, MMV688844, MMV687729, and MMV102872 and a hexadentated macrocyclic complex of copper (II) a thiosemicarbazide derivative.Figure: Roadmap to target AMR in our lab.
1. Paresh Sharma, Sonti Roy, Vasundhra Bhandari, Debabrata Dandasena and Shweta Murthy. Genetic Profiling Reveals High Allelic Diversity, Heterozygosity and Antigenic Diversity in the Clinical Isolates of the Theileria annulata from India. Front. Physiol. | doi:10.3389/fphys.2019.00673.
2. U Brahma, P Sharma, S Murthy, S Sharma, S Chakraborty, SN Appalaraju, Bhandari V. Decreased expression of femXAB genes and fnbp mediated biofilm pathways in OS-MRSA clinical isolates. Scientific reports 9 (1), 1-8.(2019)
3. Dandasena D, Bhandari V, Sreenivasamurthy GS, Murthy S, Roy S, Bhanot V, Arora J,Singh S, Sharma P. A Real-Time PCR based assay for determining parasite to host ratio and parasitaemia in the clinical samples of Bovine Theileriosis. Scientific Reports, 2018.
4. S Chakraborty, S Roy, H Mistry, N George, S Murthy, V Bhandari, P Sharma. Potential Sabotage of Host Cell Physiology by Apicomplexan Parasites for Survival Benefits. (2017).Frontiers of Immunology; 8:1261.
5. George N, Bhandari V, Reddy DP, Sharma P. (2015) Molecular and Phylogenetic analysis revealed new genotypes of Theileria annulata parasites from India. Parasit Vectors. 8:468.
6. Brahma U, Kothari R, Sharma P, Bhandari V. Antimicrobial and anti-biofilm activity of hexadentated macrocyclic complex of copper (II) derived from thiosemicarbazide against Staphylococcus aureus. Sci Rep. 2018 May 23;8(1):8050.
7. Tripathy S, Bhandari V, Sharma P, Vanjari SRK, Singh SG. Chemiresistive DNA hybridization sensor with electrospun nanofibers: A method to minimize inter device variability. Biosens Bioelectron. 2019 May 15;133:24-31. doi: 10.1016/j.bios.2019.03.031.
8. Vasundhra Bhandari, Shalini Chakraborty, Umarani Brahma, Paresh Sharma. Identification of Anti-staphylococcal and Anti-biofilm Compounds by Repurposing the Medicines for Malaria Venture Pathogen Box. Front Cell Infect Microbiol. 2018; 8: 365. Published online 2018 Oct 23. doi: 10.3389/fcimb.2018.00365.
9. Mahato S, Mistry HU, Chakraborty S, Sharma P, Saravanan R, Bhandari V. Identification of Variable Traits among the Methicillin Resistant and Sensitive Coagulase Negative Staphylococci in Milk Samples from Mastitic Cows in India. Front Microbiol. 2017; 8:1446.
10. N George, V Bhandari, DP Reddy, P Sharma. (2015) Emergence of New Genotype and diversity of Theileria orientalis parasites from bovines in India. Infection Genetics and Evolution. 36; 27-34. 4.
11. N George, V Bhandari, P Sharma (2016). Phylogenetic relationship and genotypic variability in Anaplasma marginale strains causing anaplasmosis in India. Infection Genetics and Evolution.
12. P sharma, DP Reddy, P Kumar, R Gadicherla, N George, and V Bhandari (2015). Draft genome of Staphylococcus aureus strain isolated from a clinical mastitis infected cattle. Genome announcement (2015).
13. H Mistry*, P Sharma*, S Mahato, R. Saravanan, PA Kumar, V Bhandari (2016).Prevalence and Characterization of Oxacillin susceptible mecA-positive clinical isolates of Staphylococcus aureus causing bovine mastitis in India. PLOS ONE. (2016) 11:e0162256.
14. Sharma P, Rayavara K, Ito D, Basore K, Desai SA(2015). A CLAG3 mutation in an amphipathic transmembrane domain alters malaria parasite nutrient channels and confers leupeptin resistance. Infect Immun.;83(6):2566-74.
15. Sharma P, Wollenberg K, Sellers M, Zainabdi K, Galinsky K, Nguitragool W, Neafsey D, Desai SA.(2013). An epigenetic antimalarial resistance mechanism involving parasite genes linked to nutrient uptake. J Biol Chem.,288(27):19429-40.
16. Pillai AD, Addo R, Sharma P, Nguitragool W, Srinivasan P, Desai SA.(2013).Malaria parasites tolerate broad range of ionic environments and do not require host cation remodeling. Mol Microbiol;88(1):20-34.
17. Sharma V , Sharma P , Selvapandiyan A , Salotra P. (2016). Leishmania donovanispecific Ub-related modifier-1: an early endosome-associated ubiquitin-like conjugation in Leishmania donovani. Molecular Microbiology , 99(3):597-610.
18. Wang Nguitragool, Abdullah A.B. Bokhari, Ajay D. Pillai, Kempaiah Rayavara, Paresh Sharma, Brad Turpin, L. Aravind, and Sanjay A. Desai. Malaria parasite clag genes determine nutrient uptake channel activity on infected red blood cells. Cell, 2011: 145(665– 677).
19. Vandna Bhanot, Anita Ganguly , Paresh Sharma. Haematological alterations and molecular detection of Theileriosis in crossbred cattle. Indian Journal of Animal Sciences 89(6): 21–00, June 2019/Article.
20. Gannavaram Sreenivas*, Paresh Sharma*, Robert Duncan, Poonam Salotra and Hira L.Nakhasi. Mitochondrial associated Ubiquitin fold modifier-1 mediated protein conjugation in Leishmania donovani. PLOS ONE, 20116(1), e16156. [* represent that both the authors are combined first author and have contributed equally].
21. Comparative in vivo expression of amastigote up regulated Leishmania genes in three different forms of Leishmaniasis. Paresh Sharma, Srividya Gurumurthy, Robert Duncan, Hira L.Nakhasi and Poonam Salotra. Parasitology International 2010; 59(2):262-4.
22. Srividya G, Duncan R, Sharma P, Raju BV, Nakhasi HL, Salotra P. Transcriptome analysis during the process of in vitro differentiation of Leishmania donovani using genomic microarrays. Parasitology. 2007;134:1527-39.
23. Ansari NA, Sharma P, Salotra P. Circulating nitric oxide and C-reactive protein levels in Indian kala azar patients: correlation with clinical outcome. Clin Immunol. 2007; 122(3):343-8.
24. Sanjay A. Desai, Kempaiah Rayavara, Paresh Sharma, Sayeed K. Syed, Wang Nguitragool, and Praveen Balabaskaran Nina.(2014). Membrane transport proteins as therapeutic targets in malaria. Wiley-IUBMB Malaria Book.
Shweta Nori, DST Women Scientist
MSc, MTECH, MPhil (Biotechnology) BU and RGTU, Indore Madhya pradesh
Current Research Interest: Molecular parasitology and diagnostics
Sonti Roy, PhD Student, DBT-SRF
MSc (Biotechnology), Tezpur University, Assam
Current Research Interest: Population Genetics and Host-Pathogen Interaction
Debabrata Dandasena, PhD Student, DBT-SRF
MSc (Biotechnology), IIT Rorrkee, Uttrakhand.
Current Research Interest: Functional Genomics and Post Translational Modifications
Dr Vasundhra Bhandari, DST Inspire Faculty
Current Research Interest : Anti-microbial Resistance
V. Umarani Brahma, PA-1
M.Sc (Biotechnology), Sathyabama University, Chennai.
Current Research Interest: Anti-microbial Resistance
S Akash, PhD Student, CSIR-NET JRF
MSc (Biotechnology), M.S University Of Baroda
Current Research Interest: Antimicrobial Resistance
Madhumanti Barman, PF
M.Sc (Human Physiology), Vidyasagar University, West Bengal.
Current Research Interest: Anti-microbial Resistance
K. Mohini Annapurna, PF
M.Sc Biotechnology, Krishna University, Machilipatnam,
Current Research Interest: Molecular Parasitology and Epigenetics
Sonam Kamble, PhD student, UGC-NET- JRF.
M. Sc. Biotechnology, Shivaji University Kolhapur.
Current Research Interest: Molecular Parasitology.
1. Identification of Virulence factors associated with Theileria annulata ( From DBT, India)
2. Genome-wide association study for identification of novel loci associated with resistance to Theileriosis in India. ( From DBT, India)
3. Virulence/disease pathogenesis during Bovine Mastitis ( From DBT, India)
4. Evaluation of Anti-inflammatory Natural Compounds for Therapeutic use in Mastitis of Dairy Animals. (From Ayush, India)
Room 204, 2nd Floor B Wing
National Institute of Animal Biotechnology
Survey No. 37, Opp. Journalist Colony
Extended Q City Road, Near Gowlidoddy
Gachibowli, Hyderabad
Telangana – 500032
Email: paresh[at]niab[dot]org[dot]in
Tel: +91-(0)40-2312-0144
We welcome applications from enthusiastic candidates (JRFs/SRFs/ PDF/Trainees) who are interested in research at our lab. Candidates seeking a position can write to paresh@niab.org.in with a brief CV and one-page research statement explaining how you fit in our lab and why you want to join…
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