Menu
राष्ट्रीय पशु जैव प्रौद्योगिकी संस्थान
National Institute of Animal Biotechnology
An Autonomous Institute of the Department of Biotechnology,
Ministry of Science and Technology, Government of India
राष्ट्रीय पशु जैव प्रौद्योगिकी संस्थान
National Institute of Animal Biotechnology
An Autonomous Institute of the Department of Biotechnology,
Ministry of Science and Technology, Government of India
Dr. Madhuri Subbiah is a veterinarian by profession and received her Ph.D. in Molecular Virology from University of Maryland, College Park, Maryland, USA in 2010. Dr. Subbiah previously worked as Deputy Manager, Platform Technologies, Anthem Biosciences Pvt. Ltd., Bangalore, where she was involved in managing projects from clients as well as developing novel platforms for drug development. She joined NIAB in November 2012. Her research focus at NIAB is to understand animal and poultry viral biology and to apply this knowledge for developing preventive measures and on-farm viral diagnostics.
The major threat to our livestock farming and poultry industry is from infectious diseases and viruses cause about 50% of these diseases. Animal diseases affect livestock production both directly (mortality and morbidity and loss in productivity) and indirectly (cost incurred for the prevention and control measures). Studying the animal viruses is not only essential for veterinary sciences but also for medical sciences considering the fact that 60% of all human infectious diseases recognized so far, and about 75% of emerging infectious diseases that have affected people over the past three decades, have originated from animals. In our lab, our research focus is on understanding the molecular biology of animal viruses specifically Newcastle disease virus (NDV, a poultry pathogen) and Porcine circovirus-2 (PCV-2, a porcine pathogen) to study their pathogenesis and interaction with host.
The consumption of poultry meat and egg is observed to increase every year and though the production is projected to increase, there are key challenges such as high feed costs and disease outbreaks. NDV, a paramyxovirus, causes one of the most devastating, highly contagious diseases in poultry and other avian species. The morbidity (loss in productivity) and mortality (death of the infected birds) depend on the virus strain and susceptibility of the host species. In chickens infected by virulent NDV strains, morbidity is up to 100% with 90% mortality. A recent literature has cited that between 2006 and 2009, Newcastle disease had ranked second among all livestock diseases with respect to the number of countries that were affected (Developmental and Comparative Immunology 2013, Vol 41, pages 447-453). The World Organization of Animal Health (WOAH, formerly known as OIE) has listed NDV as a notifiable disease. In countries that are NDV-free, huge money is invested for repeated testing to maintain the NDV-free status specifically for trade purposes. In developing countries like India, NDV is endemic and has enormous economic impact on both commercial and backyard poultry sectors. Though India leads in poultry meat and egg production, frequent disease outbreaks remains a challenge to the poultry industry. Our lab is actively studying the molecular epidemiology and pathogenesis of NDV in an attempt to generate better vaccines. Our lab has established the reverse genetics system, a molecular tool to engineer recombinant NDV for both studying its molecular biology as well as to generate effective vaccines.
Pig farming is a very important component in North East Region (NER) of India. According to 2019 (20th) livestock census by Government of India, more than one third of total pig population in India (9.06 million) are raised in this region. And pigs contribute to about 1.7% of the total livestock. Pig farming is a lucrative business in NER favored by their traditional custom, skill, knowledge, practice and food habits (highest monthly per capita consumption of pork is recorded in NER compared to rest of India). About 70 to 90% of tribal rural population in this area rear pigs (herd size: 1-3) as an integral part of their life. Each and every rural family in NER is known to maintain a backyard piggery unit. Piggery provides not only good source of nutrition to the population but is also sources of bristles and manure at low cost maintenance. Despite these factors, the economics of pig rearing is hit with disease outbreaks. The illnesses in pigs affect greatly their productivity and their reproductive performances. As per the latest livestock census, the total pig population has decreased by 12.0% in 2019 over 2012. The constraints are often lack of timely health care and vaccination facilities. Among the swine pathogens, the common and important viral diseases affecting pigs is PCV. While, PCV-1 is nonpathogenic, PCV-2 is associated with Postweaning Multisystemic Wasting Syndrome (PMWS), Porcine Dermatitis and Nephropathy Syndrome, proliferative and necrotizing pneumonia, reproductive failures, enteritis and porcine respiratory disease complex which are aggravated upon co-infection with other porcine viruses such as PRRSV, porcine parvovirus, Mycoplasma species. PCV-2 is known to also suppress porcine immune system. In our lab, we are studying the molecular biology of this virus with a focus to generate efficient chimeric vaccines.
1. Whole genome analysis and molecular characterization of chicken infectious anemia virus from an outbreak in a layer flock reveals circulation of genogroup IIIb in South India. Pachineella Lakshmana Rao, E Priyanka, T R Kannaki, Madhuri Subbiah. Virus Res 2022, Jan 15; 308: 198649.
2. Molecular detection and phylogenetic analysis of Marek’s disease virus virulence-associated genes from vaccinated flocks in southern India reveals circulation of virulent MDV genotype. T. R. Kannaki, E. Priyanka, Y. Nishitha, S. Vamshi Krishna, Santosh Haunshi, Madhuri Subbiah. Transboundary and Emerging diseases. 2021, https://doi.org/10.1111/tbed.14289.
3. Molecular detection and characterization of highly pathogenic porcine reproductive and respiratory syndrome virus from a natural outbreak in wild pigs, Mizoram, India. Tridib Kumar Rajkhowa, Lalnunthanga Lalnunthanga, Lalthapui Hauhnar, Doris Zodinpui, Madhuri Subbiah. Transboundary and Emerging diseases. 2021, https://doi.org/10.1111/tbed.14296.
4. Emergence of porcine circovirus 2g (PCV2g) and evidence for recombination between genotypes 2g, 2b and 2d among field isolates from non-vaccinated pigs in Mizoram, India. T.K. Rajkhowa, P.Lalnunthanga, P.L.Rao, Madhuri Subbiah, B.Lalrohlua. Infection, Genetics and Evolution. 2021, Volume 90, 104775.
5. Development and validation of high throughput Real-time Polymerase chain reaction assay for quantitative detection of Chicken Infectious Anemia Virus. T. R. Kannaki, E. Priyanka, Madhuri Subbiah, Santosh Haunshi. Virus Disease. 2021,32, pages 343–346.
6. Spectroscopic Studies on the Interaction of Naphthyridines with DNA and Fluorescent Detection of DNA in Agarose Gel. G. Mahalakshmi & B. Selvakumar & K.N. Vennila & P. Lakshmana Rao & S. Madhuri & M. Seenivasaperumal & Kuppanagounder P. Elango. Journal of Fluorescence. 2021, 31, pages327–338.
7. Structure-based design, synthesis, biological evaluation, and molecular docking of novel 10-methoxy dibenzo[b,h][1,6] naphthyridinecarboxamides. K. N. Vennila, B. Selvakumar, V. Satish, D. Sunny, S. Madhuri, K. P. Elango. Medicinal Chemistry Research. 2021, 30, pages 133–141.
8. Sophora interrupta Bedd root derived flavonoids as prominent antiviral agents against Newcastle Disease Virus. Cherukupalle Bhuvaneswar, Aluru Rammohan, Baki Vijaya Bhaskar,Pappithi Ramesh Babu,Gujjar Naveen, Duvvuru Gunasekar, Subbiah Madhuri, Pallu Reddanna and Wudayagiri Rajendra. RSC Adv., 2020, 10, 33534.
9. Molecular evolution and genetic variations of V and W proteins derived by RNA editing in Avian Paramyxoviruses. Pachineella Lakshmana Rao, Ravi Kumar Gandham & Madhuri Subbiah*. Scientific Reports. 2020, Volume 10, Article number: 9532.
10. Molecular Phylogenetics of Newcastle disease viruses isolated from vaccinated flocks during outbreaks in Southern India reveals circulation of a novel sub-genotype. Gowtham V, Ganesan V, Gopala Krishna Murthy TR, Nair S, Yegavinti N, Saraswathy PV, Suresh Kumar G, Udhayavel S, Senthilvel K, Subbiah M*. Transboundary and Emerging diseases. 2019, 66(1): 363-372.
11. Synthesis and antiviral study of novel 4-(2-(6-amino-4-oxo-4,5- dihydro-1H-pyrrolo[2,3-d]pyrimidin-3-yl)ethyl)benzamide derivatives. Selvakumar Balaraman, Nagaraj Nayak, Madhuri Subbiah and Kuppanagounder P. Elango. Medicinal Chemistry Research. 2018, 27, pages 2538–2546.
12. Design, synthesis, crystal structures and anticancer activity of 4-substituted quinolines to target PDK1. Vennila KN, Sunny D, Madhuri S, Ciattini S, Chelazzi L, Elango KP. Bioorg Chem. 2018, 81:184-190.
Nagaraj Nayak-PhD scholar
Sunny Deval-PhD scholar
Devasmita Dutta-Project fellow
Lakshmana Rao Pachinella-PhD scholar
Ruchi Malwade-PhD scholar
Subhajit Mondal, Senior Research Fellow
Kalaimagal Rajagopal, Senior Research Fellow
Industrial Project:
1. As PI: Genotyping of Newcastle Disease Virus Strains: Biological and Molecular Characterization. Funding by Globion Pvt. Ltd., Hyderabad, India. 2015-2017 (12 lakhs, 1 Project fellow for 1 yr and 3 months)
National Projects:
1. As Non-NER PI, DBT-NER Twinning: A study to understand the genetic variations among the field isolates of porcine circo viruses from piggery farms in Mizoram, with ultimate aim to develop rapid diagnostics for earlier detection of the viruses, differentiation of vaccinated and infected animals and to engineer an effective recombinant chimeric DIVA vaccine. 2016-2020 (DBT-NER Twinning, 35.888 lakhs, 1 JRF for 3 yrs) – completed in June 2020.
2. As PI, DBT Taskforce: Elucidation of the Role of Non-structural (W) Protein of Avian Paramyxoviruses. 2015-2018 (DBT taskforce, 26.40 lakhs, 1 JRF, for 3 yrs) –completed in Nov 2018.
3. As PI of Non-NER, DBT Network program on ‘Molecular platform for epidemiology, disease mapping and development of diagnostics for economically important diseases of ducks’. 2018-2021 (DBT-NER Duck program, 9 lakhs, 1 JRF for 3 yrs) – ongoing.
4. As CCPI, ICAR NASF on ‘Molecular biological studies on porcine reproductive & respiratory syndrome (PRRS) virus in pig population of North East Region of India for development of sustainable diagnostics and vaccine’. 2020-2023 (72.92 lakhs, 2 SRF for 3 yrs) – ongoing
International Projects:
1. BBSRC India Partnering Award ‘Collaboration on Diagnosis of Emerging Viruses’. 2014-2018. In collaboration with University of Nottingham, UK and TANUVAS, India – Successfully completed.
2. BBSRC India Partnering Award ‘Understanding the emergence of variant infectious bronchitis virus in chickens in UK and India: shared control strategies’. 2017-2021. In collaboration with University of Liverpool, UK and TANUVAS, India – ongoing.
NIAB Core funded Projects:
1. PI – Establishment of system for engineering recombinant NDV – completed 2019
2. PI – Understanding the upstream mechanism leading to enhanced replication of NDV by its V protein – ongoing
National Institute of Animal Biotechnology
Survey No. 37, Opp. Journalist Colony
Extended Q City Road, Near Gowlidoddy
Gachibowli, Hyderabad
Telangana – 500032
Email: madhuri[at]niab[dot]org[dot]in
Tel: +91-(0)40-2312-0143
Pubmed Link
© National Institute of Animal Biotechnology (NIAB)
Made with ❤ by NIAB