Dr. Jayant Hole completed his BVSc & AH (equivalent to DVM 2001) Nagpur Veterinary College (Dr PDKV University Akola) and M.V.Sc (Veterinary Clinical Medicine 2004) from Post Graduate Institute of Veterinary and Animal Sciences, Akola under Maharashtra Animal and Fishery Science University, Nagpur. He is registered with Veterinary Council of India (VCI) with valid registration number VCI005151 and with Maharashtra state veterinary council with valid registration number MSVC 6554 respectively. He has also completed a Certificate Course from the International Council for Laboratory Animal Science (ICLAS 2012) through the CSIR – Institute of Genomics and Integrative Biology, New Delhi, the Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA, Government of India, Utrecht University, the Netherlands. Dr. Hole has also undergone further training at national and international levels through workshops and symposia on animal facility establishment, colony management, breeding strategies, Good Manufacturing Practices (GMP), and Good Laboratory Practices (GLP).

Basic Information
The state-of-the-art barrier-maintained laboratory animal facility at National Institute of Animal Biotechnology is established to conduct experiments using rodents and lagomorphs. It is with clean and dirty (service corridor concept and built in compliance with the guidelines of Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA). The total area spread over two floor approx. 975 Square meter. The experiments conducted on animals at this facility are through the approval of Institutional Animal Ethics committee (IAEC). Russell and Burch’s concept of application of 3R Reduction, Refinement and Replacement in all experiments on animals are closely observed. All the utilities viz. chillers, AHU, compressor, soft water plant and electrical control panel for heavy equipment are planned on terrace area with the ease of maintenance personnel movement without entering inside the facility.

Objectives and Key features
The facility is a core scientific service department of the Institute with an objective to breed, supply and procure, raise experimental laboratory animals of high quality and sterile conditions. It is focused to provide husbandry, enrichment, nutrition, veterinary care, technical and professional support to the scientific community of the Institute to facilitate research on animals. Defined barrier practices are followed strictly. The facility is under electronic surveillance (CCTV system). Access to facility is through access cards only for those who are authorized persons it helps to minimize the risk of infection to the animal’s colonies. All the parameters like temperature humidity, air velocity pressure and running of AHU are monitored and controlled through BMS (Building monitoring system). Dark and light cycle 12:12 hours for the normal physiological behaviour of the animals is maintained through automated DALI (Digitally accessible lighting interface) control system. Suitable nutrition with appropriate nutrients, water quality and sterilization using high pressure high vacuum autoclaves are used on routine basis to maintain sterile conditions in animal colonies.

This facility is operational from August 2019 and conducting IAEC approved protocols for the experiments on animals. Presently this facility housed inbred strains Balb/c, C57/BL6, CBA/J, FVB, NOD SCID, Neo R Tg line, outbred CD1 and Wistar Rats and Rabbits. All records are properly maintained related to breeding and experiments using registers and software tools. The facility is registered with Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA), Ministry of Environment, Forests and climate change, Government of India Registration N.2063/GO/RBi/SL/19/CPCSEA dated 10.04.2019.

Dr. Hole has more than a decade 15 years of experience in veterinary science and laboratory animal medicine. He has established animal facilities and managed colonies of laboratory rodents and lagomorphs (including transgenic, knock-out, athymic and severe combined immunodeficient strains) and larger species of bovine, caprine, porcine and canine. Dr. Hole has worked with various organizations on various areas of animal testing potency, safety and challenge studies along with maintaining animal breeding program and health and genetic monitoring program for the proven strains for quality of animals. He contributed briefly in the area of vaccine testing for FMD, HS, BQ, ET, Brucella and Rabies for quality control biological and production of live/killed and cell culture based poultry vaccine. He has been part of GMP, GLP, CPCSEA and American Association for Laboratory Animal Care (AALAC) accreditation of animal facilities and Occupational Health and Safety program at various organization.

Before joining NIAB, Dr. Hole worked at the Centre for DNA Fingerprinting and Diagnostics (CDFD 2011-2017), Hyderabad, where he established CDFD Experimental Animal Facility withgoal and objective to develop this facility at par world class state-of-the- art facility to scientifically support high standard research on laboratory animals. Objective to target to set the ethical standards of the experiments on animals, Animal welfare, Human and Animal Interest set points of the experiments.

Dr Hole joined NIAB on June 2017, heading the Animal House Facility department at NIAB and overall key person for day to day operations, its establishment and operationalization. The responsibilities include breeding, management, husbandry of genetically defined and mutant laboratory animals, as well as their supply for experiments, facilitating experiments on animals including conduct of procedures / surgeries on animals etc., Handling documentation and duties related to import of animals, and active participation in the Institutional Animal Ethics Committee.

1. CPCSEA nominee for Institutional Animal Ethics Committees, and for animal house inspections

2. Certification on Scientific Efficiency for veterinary vaccine manufacturing, toxicity, bioassay and testing, Food and Drug administration (FDA), 2008

3. Member – Laboratory Animal Scientists Association (LASA), Indian Society for the Advancement in Veterinary Clinical and Preventive Medicine (ISVM), Laboratory Animal Scientists Association of India (LASAI)

4. Executive committee member of Laboratory animal scientist association of India

1. Training on facility planning, logistic and technological solutions for laboratory animal facilities, FIRC Institute of Molecular Oncology Foundation – European Institute of Oncology, Milano, Italy, 2012

2. Attended the Federation of Laboratory Animal Science Associations (FELASA) Category-C recommended International Course on Laboratory Animal Science, 2013

3. Training on Animal welfare, CPCSEA guidelines and role of CPCSEA Nominee” organized by NIAW and CPCSEA at Ballabhgara, Haryana during 7th -11th July2014

4. Clean Mouse facility training at (NCBS) National Centre for Biological sciences, Bangalore from 10th -18th April2012

5. Trained in Laboratory Animal Facility management, care and specialized breeding strategies, molecular and transgenic techniques at (CCMB) Centre for Cellular and Molecular Biology, Hyderabad during 13th-17th May2011

6. Good Manufacturing Practices Compliance and Quality Management System in the 21st July to 22 August 2009 at Haryana

7. LYOPHILIZATION training IMA EDWARD, Lyofast 10 Machine for lyophilizationtechnique during 15th -29 December 2009

8. Business English Certificate Training, British Council University of Cambridge training on 01 -19 Nov.2007

9. Leadership Development Programmed Training at Lonavala India on July 10-13,2007

10. Laboratory Animal Science Training from National Centre for Laboratory Animal Science, 6-10 Nov 2006 at National Institute of Nutrition,Hyderabad

11. Attended GMP and GLP for Products Development Training conducted on 11th -12th August 2006 at Pune

12. Good Laboratory Practices Training on July 21 ,2005, at Pune

13. Laboratory Animal Science and Medicine Induction training at NIV and NCCS, Pune Aug2004-Jan2005

14. Computer information technology and language coarse training year 2002

1. 9^th International conference of LASA India “laboratory animals in biomedical research – the way forward” November 21 – 23, 2019 at IISER, PUNE

2. 8^th AFLAS congress, November 29-December 01, 2018, Bangalore, India

3. 8^th International conference of LASA India, November 25-26, 2017 on “Recent Advances in 3R’s and Laboratory Animal Science” at JNTUM New Delhi

4. International conference on “Advances in Laboratory Animal Science for Modeling Human diseases” at Banglore during 14th-15th October,2016.

5. Animal Welfare and Alternatives to Experimentation in Biomedical Research, World Laboratory Animal Day, National Institute of Nutrition, Hyderabad, 2016

6. Animals in Research and Tastings-A cross talk between relevance and Ethics, CSIR-Central Drug Research Institute, Lucknow, 2015

7. Recent Practices for the Improvement of Animal Welfare, World Laboratory Animal Day, National Institute of Nutrition, Hyderabad, 2015

8. Promotion of Animal Research, Welfare and Harmonization of Laboratory Animal Science, Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Navi Mumbai, 2015

9. Alternatives to Animals in Research, CPCSEA, Ministry of Environment and Climate Change, Government of India, New Delhi, 2014

10. Laboratory Animal Medicine and Management, LASA and CPCSEA, Hyderabad, 2013

11. Animal Heath, Well-being and Quality – The role of IAEC, LASAI & CPCSEA, Hyderabad, 2012

12. Health Monitoring of Laboratory Animals, Indian Institute of Science, Bangalore, 2012

13. Conference on Current Perspectives and Future Challenges in Laboratory Animal Management, Bangalore, 2007

14. Rational use of Animals in Research, Hyderabad, 2006

Animal House Facility
National Institute of Animal Biotechnology
Survey No. 37, Opp. Journalist Colony
Extended Q City Road, Near Gowlidoddy
Gachibowli, Hyderabad
Telangana – 500032

Email: jayant[at]niab[dot]org[dot]in
Tel: +91-(0)40-2312-0161/163
M: +91 – 8121138971/ 9963305790