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राष्ट्रीय पशु जैव प्रौद्योगिकी संस्थान
National Institute of Animal Biotechnology
An Autonomous Institute of the Department of Biotechnology,
Ministry of Science and Technology, Government of India
राष्ट्रीय पशु जैव प्रौद्योगिकी संस्थान
National Institute of Animal Biotechnology
An Autonomous Institute of the Department of Biotechnology,
Ministry of Science and Technology, Government of India
Dr. Anand Srivastava received Bachelors in Science (B.Sc.) from University of Delhi, Delhi, and Masters of Science (M.Sc-Biotechnology) from the Allahabad University, Allahabad, India. He did Ph.D. from Jawaharlal University (JNU) working at International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India. He then worked at Institut Pasteur, Paris (2008-2011) and Institut National de Transfusion Sanguine, Paris (2011-2012) as a Post-doctoral Fellow. He joined National Institute of Animal Biotechnology (NIAB) in 2013.
Selected awards, honors and fellowships:
Membership of Societies:
Area of Specialization: Host parasite interaction, Proteomics, Drug discovery, Yeast 2 hybrid, Phage display
Our group broadly focus on infection disease specifically with reference to ticks and tick borne diseases (TTBDs). Ticks are obligate haematophagous ectoparasites of wild and domestic animals as well as humans. Ticks are considered to be the second largest arthropod vectors for transmitting diseases in humans and animals. The tick-borne diseases of humans include Rocky Mountain spotted fever (RMSF), anaplasmosis, human ehrlichiosis, Mediterranean spotted fever, Lyme disease/lyme borreliosis, Crimean-Congo hemorrhagic fever, Tick paralysis, Tick toxicoses etc. The diseases transmitted by tick in domestic and wild animals include babesiosis, theileriosis, bovine anaplasmosis, cowdriosis etc. We are working on development of interventions for ticks and theileriosis. Apart from TTBDs we are interested in understanding the immunology of the ruminants. A brief description of the specific areas of research are outlined below:
Tick-borne disease (Theileriosis): Theileriosis is caused by the apicomplexan parasite Theileria. This is a unique parasite as it causes unchecked proliferation of the leucocytes, thus leading to cancer like phenotype in the host cells. The untreated cattle die in 3-4 weeks. The present vaccine and drug molecules have their own limitations. Hence we are in the quest of developing better interventions in the form of vaccine and drug molecules for curing theileriosis. Currently we are in the process of identifying newer targets for vaccine development and working on identification of new drug molecules. In order to develop newer drug candidates my research group is interested in the identification of a key molecule(s) from parasite which is essential for the transformation of the host cells. Further, in order to develop vaccine, we have identified various surface molecules which can be targeted for vaccine development.
Ticks: We are also working towards the development of formulations with plant extracts as anti-tick. We have screened various plants extracts with anti-tick property. We have obtained various leads and are working towards making formulations. These formulations will be tested in vivo and will be commercialized in future.
Immunology of ruminants: B cells play an important role in protection of animals from various infectious agents. B cells comprise of various subsets with specific roles. Most of the knowledge about B cell and its subsets has been obtained from the studies in mice and humans. In case of ruminants, the information about the subclasses of B cells is very limited. A precise identification and phenotypic characterization of ruminants’ B cell subsets is highly important for both basic and translational research. We aim to develop monoclonal antibodies against those CD markers of bovine/ovine B cell subsets for which reagents are not commercially available. We would like to estimate the level of B cell subsets in various tissues such as lymph node, cord blood and PBMCs in ovine species. This study will open up new avenues in the ruminant B cell biology by providing information about ruminant B cell subsets.
2022
30. Araveti PB, Kar PP, Kuriakose A, Sanju A, Kumar KA, Srivastava A. Identification of a novel interaction between Theileria Prohibitin (TaPHB-1) and bovine RUVBL-1. Microbiology Spectrum. 2023 10.1128/spectrum.02502-22
29. Putty K, Rao PL, Vishweshwar Kumar Ganji, Devasmita Dutta, Subhajit Mondal, Nagendra Hegde, Anand Srivastava, Madhuri Subbiah. The first report on the complete genome sequence of Lumpy skin disease virus in India. 2023 Virus gene
28. Kar, P.P., Araveti, P.B., Kuriakose, A. Srivastava A. Design of a multi-epitope protein as a subunit vaccine against lumpy skin disease using an immunoinformatics approach. Sci Rep 12, 19411 (2022). https://doi.org/10.1038/s41598-022-23272-z
27. Kar PP, Araveti PB, Srivastava A. Deciphering the kinome of Theileria annulata for identification of drug targets and anti-theilerial drug. Ticks Tick Borne Dis. 2022 Sep 29;13(6):102049.
doi: 10.1016/j.ttbdis.2022.102049. PMID: 36215767.
26. Singh D, Patri S, Narahari V, Segireddy RR, Dey S, Saurabh A, Macha V, Prabhu NP, Srivastava A, Kolli SK, Kota AK. A Conserved Plasmodium Structural Integrity Maintenance Protein (SIMP) is associated with sporozoite membrane and is essential for maintaining shape and infectivity. Molecular microbiology 17 March 2022 https://doi.org/10.1111/mmi.14894
25. Araveti PB, Vijay M, Kar PP, Varunan S, Srivastava A. MMV560185 from Pathogen box induces extrinsic pathway of apoptosis in Theileria annulata infected bovine leucocytes International Journal for Parasitology: Drugs and Drug Resistance Volume 18, April 2022, Pages 20-31
https://doi.org/10.1016/j.ijpddr.2021.12.003
2021
24. Gulla S, Reddy VC, Araveti PB, Lomadad D, Srivastava A, ReddyaKakarl MC, Reddy R. Synthesis of titanium nanotubes conjugated with quercetin and its in-vivo anti-tumor activity against skin cancer. Journal of Molecular Structure (2021) Volume 1249, 5 February 2022, 131556 https://doi.org/10.1016/j.molstruc.2021.131556
23. Acaricidal activity of Annona squamosa L. seeds extracts against cattle tick, Rhipicephalus microplus. Jadhav ND, Rajurkar SR, Vijay M, Narladkar BW, Srivastava A, Mamde CS, Vaidya MS, Chigure GM, Kumar S. International Journal of Tropical Insect Science (2021). https://doi.org/10.1007/s42690-021-00630-4
22. Gulla S, Lomada D, Araveti PB, Srivastava A, Murikinati MK, Reddy KR, Inamuddin, Reddy MC & Altalhi T. Titanium dioxide nanotubes conjugated with quercetin function as an effective anticancer agent by inducing apoptosis in melanoma cells. Journal of Nanostructure in Chemistry (2021). https://doi.org/10.1007/s40097-021-00396-8
2019
21. Araveti, P.B., Srivastava, A. Curcumin induced oxidative stress causes autophagy and apoptosis in bovine leucocytes transformed by Theileria annulata. Cell Death Discov. 5, 100 (2019). https://doi.org/10.1038/s41420-019-0180-8 ( *corresponding author)
20. Gangnard S, Chêne A, Dechavanne S, Srivastava A, Avril M , Smith JD. VAR2CSA binding phenotype has ancient origin and arose before Plasmodium falciparum crossed to humans: implications in placental malaria vaccine design Scientific reports 9, 16978 (2019). https://doi.org/10.1038/s41598-019-53334-8
19. Dorin-Semblat D, Tétard M, Claës A, Semblat JP, Dechavanne S, Fourati Z, Hamelin R, Armand F, Matesic G, Nunes-Silva S, Srivastava A, Gangnard S, Lopez-Rubio JJ, Moniatte M, Doerig C, Scherf A, Gamain B. Phosphorylation of the VAR2CSA extracellular region is associated with enhanced adhesive properties to the placental receptor CSA Plos Biology 2019 https://doi.org/10.1371/journal.pbio.3000308
2018
18. PP Kar, A Srivastava* Immuno-informatics Analysis to Identify Novel Vaccine Candidates and Design of a Multi-Epitope Based Vaccine Candidate Against Theileria parasites Frontiers in Immunology 9 2018 ( * corresponding author) DOI=10.3389/fimmu.2018.02213
17. Chêne A, Gangnard S, Dechavanne C, Dechavanne S, Srivastava A, Tétard M, Hundt S, Leroy O, Havelange N, Viebig NK and Gamain B Down-selection of the VAR2CSA DBL1-2 expressed in E. coli as a lead antigen for placental malaria vaccine development 3(28) 2018 NPJ Vaccine https://doi.org/10.1038/s41541-018-0064-6
2017
16. Anilkumar K, Reddy GV, Azad R, Yarla NS, Dharmapuri G, Srivastava A, Kamal MA, and Reddanna Pallu. Evaluation of Anti-Inflammatory Properties of Isoorientin Isolated from Tubers of Pueraria tuberosa. Oxidative Medicine and Cellular Longevity Volume 2017: 2017:5498054. | https://doi.org/10.1155/2017/5498054
2016
15. de Moraes LV, Dechavanne S, Sousa PM, Barateiro A, Cunha SF, Nunes-Silva S, Lima FA, Murillo O, Marinho CR, Gangnard S, Srivastava A, Braks JA, Janse CJ, Gamain B, Franke-Fayard B, Penha-Gonçalves C. A Murine Model for Pre-Clinical Studies on Var2CSA-Mediated Pathology Associated to Malaria in Pregnancy. Infect Immun. 2016 Apr 4. pii: IAI.01207-15.
2015
14. Gangnard S, Lewit-Bentley A, Dechavanne S, Srivastava A, Amirat F, Bentley GA, Gamain B. Structure of the DBL3X-DBL4ε region of the VAR2CSA placental malaria vaccine candidate: insight into DBL domain interactions. Sci Rep. 2015 Oct 9;5:14868.
13. Dechavanne S, Srivastava A, Gangnard S, Nunes-silva S, Dechavanne C, Fievet N, Deloron P, Chêne A, Gamain B The parity-dependent recognition of the DBL1X-3X suggests an important role of the VAR2CSA high affinity CSA-binding region in the development of the humoral response against placental malaria. Infect Immun. 2015 Mar 30. pii: IAI.03116-14
2014
12. Gullingsrud J, Milman N, Saveria T, Chesnokov O, Williamson K, Srivastava A, Gamain B, Duffy PE, Oleinikov AV. High-Throughput Screening Platform Identifies Small Molecules That Prevent Sequestration of Plasmodium falciparum-Infected Erythrocytes. J Infect Dis. 2014 Oct 29. pii: jiu589
2011
11. Srivastava A, Gangnard S, Dechavanne S, Amirat F, BentleyGA, Bentley GA and Gamain B Var2CSA minimal CSA binding region is located within the N-terminal region. PLoS One 2011 May 11, 6(5):e20270.
10. Rathore S, Jain S, Sinha D, Gupta M, Asad M, Srivastava A, Narayanan MS, Ramasamy G, Chauhan VS, Gupta D, and Mohmmed A. Disruption of a mitochondrial protease machinery in Plasmodium falciparum is an intrinsic signal for parasite cell death. Cell Death and Disease 2011 Nov 24;2:e231
9. Avril M, Hathaway MJ, Srivastava A, Dechavanne S, Hommel M, Beeson JG, Smith JD, Gamain B. Antibodies to a Full-Length VAR2CSA Immunogen Are Broadly Strain-Transcendent but Do Not Cross-Inhibit Different Placental-Type Parasite Isolates. PLoS ONE 2011 Feb 4;6(2):e16622
8. Pandey A, Srivastava A, Pandey A. Acidithiobacillus ferrooxidans: A bioleaching bacteria for better iron(II) oxidation ability International Journal of Innovation in biological and Chemical Sciences, 1, 32-37, 2011.
2010
7. Srivastava A, Singh S, Dhawan S, Alam MM, Mohmmed A, Chitnis CE. (contributed to cover pager image) Localization of Apical Sushi Protein in Plasmodium falciparum merozoites. Mol Biochem Parasitol. 2010 Nov;174(1):66-9
6. Srivastava A, Gangnard S, Round A, Dechavanne S, Juillerat A, Raynal B, Faure G, Baron B, Ramboarina S, Singh SK, Belrhali H, England P, Lewit-Bentley A, Scherf A, Bentley GA, Gamain B. Full-length extracellular region of the var2CSA variant of PfEMP1 is required for specific, high-affinity binding to CSA. PNAS 2010 Mar 16;107(11):4884-9
2009
5. Mayor A, Rovira-Vallbona E, Srivastava A, Sharma SK, Pati SS, Puyol L, Quinto L, Bassat Q, Machevo S, Mandomando I, Chauhan VS, Alonso PL, Chitnis CE. Functional and immunological characterization of a Duffy Binding-Like alph1 a domain from Plasmodium falciparum-erythrocyte membrane protein-1 that mediates resetting. Infect Immun. 2009 Sep;77(9):3857-63
2008
4. Korde R, Bhardwaj A, Singh R, Srivastava A, Chauhan VS, Bhatnagar RK, Malhotra P. A prodomain peptide of plasmodium falciparum cysteine protease (falcipain-2) inhibits malaria parasite development. J Med Chem. 2008 Jun 12;51(11):3116-23.
2007
3. Sivakumar S, Rajagopal R, Raja Venkatesh G, Srivastava A, Bhatnagar RK. Knockdown of aminopeptidase-N from Helicoverpa armigera larvae and in transfected SF21 cells by RNA interference reveals its functional interaction with Bacillus thuringiensis insecticidal protein Cry1Ac. J Biol Chem. 2007 Mar 9;282(10):7312-9
2004
2. Kulkarni KA, Srivastava A, Mitra N, Sharon N, Surolia A, Vijayan M, Suguna K. Effect of glycosylation on the structure of Erythrina corallodendron lectin. Proteins. 2004 Sep 1; 56(4):821-7.
1. Jeyaprakash AA, Srivastav A, Surolia A, Vijayan M. Structural basis for the carbohydrate specificities of artocarpin: variation in the length of a loop as a strategy for generating ligand specificity. J Mol Biol. 2004 May 7; 338(4):757-70.
Book Chapter
1. Anand Srivastava, Yves Durocher, and Benoit Gamain Expressing full length PfEMP1 proteins in the HEK293 expression system, Malaria: Methods and Protocols, Series Methods in Molecular biology. Ed Robert Menard 2nd ed. 2013;923:307-19. Humana Press (ISBN 978-1-62703-025-0)
Pubmed Link
Pubmed Dr. Anand Srivastava
Google Scholar Link
https://scholar.google.co.in/citations?user=5bnHpccAAAAJ&hl=en
Present Members
ARAVETI PRASANNA BABU- PhD scholar
PRAJNA PARIMITA KAR- PhD scholar
M RAJITHA- PhD scholar
VIJAY MACHA-PhD scholar
AMAR PRAJAPATI- PhD Scholar ICMR-JRF
KRISHNAGAANTH M – PhD Scholar DBT-JRF
ACHINTYA SANJU – Project Associate I
RAHUL YADAV- Project Associate II
Past Lab Members
1. Akshay Kuriakose-Project JRF
2.Dr. (Mrs) Rinky Sharma Mukherji(Project Fellow 2013-14)
3. Ms. Rakhi Harne (Project Fellow 2014-15)
4. Ms. Swetha Murthy(SRF 2014-2016)
5. Dr. Anil Kumar(Research Associate 2015)
6. Mr. Prasanna Babu(Project Fellow 2015-16)
7. Ms. Rolly Kumari(Project Fellow 2016-2017)
8. Dr. (Mrs) Mathu Varunan Shalu(N-PDF 2017-19)
9. Dr. Amit Kumar Sahu(Research Associate 2017-2018)
10. Mr. Shiga Nagaraju(Lab Technician July 2017)
11. Dr. (Mrs) Soma Behera(Research Associate 2019-20
Ongoing projects
1. Evaluation of medicinal plant extracts for anti-tick activity and identification of active compounds Ministry of AYUSH, National Medicinal Plants Board (New Delhi, India) 2017-04 to 2020-04
Grant: R&D/TL-01/2017
2. Establishment of genome manipulation technology in Theileria parasite for identification of genes involved in transformation of host cell. 2019-2021
Grant: BT/PR17590/GET/119/271/2018
Completed project
Elucidation of mechanism(s) of transformation of host cells by Theileria annulata Science and Engineering Research Board (Delhi, India) 2017-03 to 2020-03
Grant: EMR/2015/001828/AS
National Institute of Animal Biotechnology
Survey No. 37, Opp. Journalist Colony
Extended Q City Road, Near Gowlidoddy
Gachibowli, Hyderabad
Telangana – 500032
Email: anand[at]niab[dot]org[dot]in
Tel: +91 40 2312 0141
My lab is currently open for the motivated and hardworking research scholars and post-doctoral researchers.
For PhD position: If you have NET-JRF and you are interested in my research areas you may contact me for further discussion.
For Post-doctoral position: If you have new ideas which is the lines with my lab interest kindly contact me for discussion for applying to various granting agency for fellowship.
All the available positions in extramural grants are displayed on the NIAB website.
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