The state-of-the-art barrier-maintained laboratory animal facility at National Institute of Animal Biotechnology is established to conduct experiments using rodents and lagomorphs. It is with clean and dirty (service corridor concept and built in compliance with the guidelines of Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA). The total area spread over two floor approx. 975.065 Square meter plinth area. The experiments conducted on animals at this facility are through the approval of Institutional Animal Ethics committee (IAEC). Russell and Burch’s concept of application of 3R Reduction, Refinement and Replacement in all experiments on animals are closely observed. All the utilities viz. chillers, AHU, compressor, soft water plant and electrical control panel for heavy equipment are planned on terrace area with the ease of maintenance personnel movement without entering inside the facility.
Animal research and animal research facilities are critical to the biomedical research enterprise. Animal species are used in every stage of the research and development effort-from discovery, to development and safety testing, to clinical trials, and to manufacture-because their biological systems, genetic structures, and immunological responses, in various ways, closely mirror ours as a species which articulated the importance and the value of animal research.
The animal research facility, also known as the vivarium, is a specially designed building type, which accommodates exquisitely controlled environments for the care and maintenance of experimental animals. Animal research facilities are related to but distinct from research laboratories. The facilities are complex, and critical to build and to operate and maintain, but they are vital to the support of a proper, safe, and humane research effort for the betterment of human and animal health. Research using animals is sensitive, and the design of these facilities requires a heightened awareness to the issues of biosecurity concern to maintain high quality animals and throughput research outcomes.
Objectives and Key features
The facility is a core scientific service department of the Institute with an objective to breed, supply, procure and raise experimental laboratory animals of high quality under clean and sterile conditions. It focused to provide husbandry, enrichment, nutrition, veterinary care, technical and professional support to the scientific community of the Institute to facilitate research on animals where animal welfare and human handling ensures that the establishment and the fellows minimize the possibility of needless handling, stress ad discomfort to the animals housed Defined barrier practices are followed strictly. The facility is under electronic surveillance.
(CCTV system). Access to facility is through access cards only for those who are authorized persons it helps to minimize the risk of infection to the animal’s colonies. All the parameters like temperature humidity, air velocity pressure and running of AHU are monitored and controlled through BMS (Building monitoring system). Dark and light cycle 12:12 hours for the normal physiological behaviour of the animals is maintained through automated DALI (Digitally accessible lighting interface) control system. Suitable nutrition with appropriate nutrients, water quality and sterilization using High pressure high vacuum autoclaves on routine basis are the key highlights to maintain sterile conditions in animal colonies.
This facility is operational from August 2019 and conducting IAEC approved protocols for the experiments on animals. Presently this facility housed inbred strains Balb/c, C57/BL6, CBA/C3J, NOD SCID, FVB NeoR Trasngenic strain line, outbred CD1 mouse line and outbreed Wistar Rats are maintained, in addition out breed New Zealand white Rabbits are frequently being used for the generation of antibodies and other experiments. All records are properly maintained related to breeding and experiments using registers and software tools. The facility is registered with Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA), Ministry of Environment, Forests and climate change, Government of India dated 10.04.2019.
Maintaining Good Laboratory Practices (GLP) for the Animal Facility, intended to assure quality of animals and safety of personnel involved in biomedical and behavioural research. These include: Supervision of animal nutrition; Surveillance, diagnosis, treatment and control of disease in the colonies of animals housed in the Facility. Being a centralised research resource facility provide comprehensive research support to the researchers including whole body animal imaging for rodents, stereo zoom microscope for surgical procedure and other assistance needed for animal research.